Simultaneous Determination of Syringin and Pedunculoside in Zhuang Medicine Yuyang Powder by HPLC
10.6039/j.issn.1001-0408.2016.06.31
- VernacularTitle:HPLC法同时测定壮药愈疡散中紫丁香苷和长梗冬青苷的含量
- Author:
Yanhua FENG
;
Jieping QIN
;
Zhipiao HUANG
;
Jiemei QIN
;
Miao WANG
;
Pengfei LIU
- Publication Type:Journal Article
- Keywords:
Syringin;
Pedunculoside;
Content determination;
HPLC;
Zhuang medicine Yuyang powder
- From:
China Pharmacy
2016;(6):818-820
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of syringinand and pedunculoside in Zhuang medicine Yuyang powder. METHODS:HPLC was performed on the column of Agilent ODS with mobile phase of acetonitrile-water (gradient elution)at a flow rate of 1 ml/min,the detection wavelength was 210 nm,the column temperature was 30℃,and the in-jection volume was 5μl. RESULTS:The linear range was 0.71-3.55μg for syringin(r=0.999 7)and 1.62-8.10μg for pedunculoside (r=0.999 8), respectively;RSDs of precision, stability and reproducibility tests were lower than 3%;recoveries were 100.8%-104.9%(RSD=1.7%,n=6) and 96.0%-100.8%(RSD=2.2%,n=6). CONCLUSIONS:The method is simple,stable and reproducible,and can be used for the simultaneous determination of syringinand and pedunculoside in Zhuang medicine Yuyang powder.
