Study on the Quality Standard of Ganmao Shangfengke Tea
10.6039/j.issn.1001-0408.2016.12.40
- VernacularTitle:感冒伤风咳茶的质量标准提高研究
- Author:
Qing LIN
;
Xiaoying FU
;
Zhixiu LIN
- Publication Type:Journal Article
- Keywords:
Ganmao shangfengke tea;
TLC;
Honokiol;
Magnolol;
HPLC;
Content determination
- From:
China Pharmacy
2016;27(12):1705-1707
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To improve the quality standard for Ganmao shangfengke tea. METHODS:TLC was used to identify the Saposhnikovia divaricata and Glycyrrhizae Radix et Rhizoma in the preparation. HPLC was used to determine the content of magnolol and honokiol:the column was Phenomenex Luna C18 with mobile phase of methanol-water(77:23,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 294 nm,column temperature was room temperature,and the injection volume 5 μl. RE-SULTS:The TLC spots of S. divaricata and Glycyrrhizae Radix et Rhizoma were clear and well separated. The linear range was 3.13-100.2 μg(r=0.9999) for magnolol and 3.05-97.6 μg(r=0.9999) for honokiol;RSDs of precision,stability and accuracy tests were lower than 2%;recoveries were 98.2%-99.5%(RSD=0.5%,n=6)and 98.6%-99.6%(RSD=0.4%,n=6),respective-ly. CONCLUSIONS:The improved standard can be used for the quality control of Ganmao shangfengke tea.
