Analysis of the Impact of Ethanol Volume Fraction on Extract Rate of Ginsenoside Monomer by Q-TOF LC/MS
10.6039/j.issn.1001-0408.2015.34.27
- VernacularTitle:Q-TOF LC/MS技术分析乙醇体积分数对人参单体皂苷提取率的影响Δ
- Author:
Zonglin YANG
;
Hui LI
;
Bing WANG
;
Shuying LIU
- Publication Type:Journal Article
- Keywords:
Ethanol volume fraction;
Panax ginseng;
Q-TOF-LC/MS;
Saponin monomer;
Extract rate
- From:
China Pharmacy
2015;(34):4834-4836
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the impact of ethanol volume fraction on the extract rate of ginsenoside monomer. METH-ODS:Water and different volume fraction of ethanol solution(20%,50%,70%,95%,V/V)were used for ginseng extract. High performance liquid chromatography-quadrupole-time of flight-mass spectrometry (Q-TOF LC/MS) was applied to identify compo-nents attributable of each peak. Especially 16 kinds of main saponins were analyzed. RESULTS:The volume fraction of ethanol had a significant impact on extract rate of ginsenoside. When the volume fraction of ethanol was 70%,extract rate of ginsenoside was the highest;however,the differences were mainly caused by protopanaxatriol saponins. The yield of protopanaxadiol ginsen-osides was similar in different volume fraction of ethanol solution. CONCLUSIONS:When the volume fraction of ethanol is differ-ent,result is not only different in the total saponin,but also different in the proportion of the monomers.
