Study on Inhibitory Effects of Triptolide on the Proliferation of Fibroblast-like Synovial Cells from Pa-tients with Rheumatoid Arthritis in vitro
10.6039/j.issn.1001-0408.2015.31.13
- VernacularTitle:雷公藤甲素对类风湿关节炎患者滑膜成纤维样细胞增殖的体外抑制作用研究
- Author:
Lei YE
;
Shuang JIA
;
Wenping PAN
- Publication Type:Journal Article
- Keywords:
Rheumatoid arthritis;
Fibroblast-like synovial cells;
Triptolide;
Proliferation;
Inhibitory;
Mechanism
- From:
China Pharmacy
2015;26(31):4357-4359
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of triptolide(TP)on the proliferation of fibroblast-like synoviocytes(FLS) from patients with rheumatoid arthritis(RA)in vitro. METHODS:5 RA patients received knee arthroplasty or synovectomy to ob-tain synovial tissue. FLS was isolated,cultured and identified,and then incubated in the presence of TP [0 (blank control),50, 100 and 200 nmol/ml] for 24,48 and 72 h. The effects of TP on FLS was evaluated by MTT,and then proliferation inhibitory rate was calculated;flow cytometry was used to detect the apoptosis and cell cycle of FLS. RESULTS:The inhibitory rates of TP(50, 100 and 200 nmol/ml)on the proliferation of FLS were 17.46%-52.56%,which was positively correlated with drug concentration. Compared with blank control group,100 and 200 nmol/ml TP could increase the percentage of cells in G0/G1 phase and decrease the percentage of cells in S phase,with statistical significance(P<0.05);200 nmol/ml TP could induce cell apoptosis. CONCLU-SIONS:TP could inhibit the proliferation and also could induce the apoptosis of FLS in RA patients in vitro,which may be one of its mechanism for treating RA.
