Analysis of the Genetic Diversity of Germplasm Resources of Duchesnea indica from Hunan by Random Amplified Polymorphic DNA
10.6039/j.issn.1001-0408.2015.19.11
- VernacularTitle:随机扩增多态性DNA技术对湘产蛇莓种质资源的遗传多样性分析Δ
- Author:
Xiangdan LIU
;
Yu GAO
;
Yali ZHANG
;
Jialuo CAI
;
Qiaozhen TONG
;
Congzi ZHANG
- Publication Type:Journal Article
- Keywords:
Duchesnea indica;
Germplasm resources;
Random amplified polymorphic DNA;
Genetic diversity;
Clustering analysis;
Polymerase chain reaction
- From:
China Pharmacy
2015;(19):2628-2631
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the genetic diversity of germplasm resources of Duchesnea indica from Hunan. METHODS:The random amplified polymorphic DNA(RAPD)reaction system of germplasm resources was established and RAPD-PCR was ad-opted to detect the expressions of 24 groups of D. indica from Hunan(including 21 wild and 3 cultivated varietos in different geo-graphical distribution). Agarose gel electrophoresis was used to analyze the results and calculate allele number (Na),effective al-lele number (Ne) the polymorphism points percentage (PPB),Nei’s gene diversity index (H) and Shannon’s information index (I). RESULTS:Totally 14 polymorphic primers were screened and 90 electrophoresis bands were amplified,including 81 polymor-phic bands with Na of 1.900 0,Ne of 1.540 1,PPB of 90.0%,H of 0.312 8 and I of 0.467 5. CONCLUSIONS:The genetic diver-sity of D. indica from Hunan is rich,and there were differences in genetic background between the cultivated species and wild spe-cies;clustering results show significant correlation with geographic distribution.
