Content Determination of Calceolarioside A in the Leaves of Fraxinus mandschurica by HPCE
10.6039/j.issn.1001-0408.2015.33.35
- VernacularTitle:HPCE法测定水曲柳叶中荷包花苷A的含量
- Author:
Yujuan CHEN
;
Wei WANG
;
Chengyu LI
;
Jiajia ZHANG
;
Ye YANG
;
Bubu LI
- Publication Type:Journal Article
- Keywords:
Fraxinus mandschurica;
Leaf;
Calceolarioside A;
HPCE
- From:
China Pharmacy
2015;26(33):4709-4710
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of calceolarioside A in the Leaves of Fraxi-nus mandschurica. METHODS:HPCE was performed on the capillary of uncoated fused silica capillary,buffer solution of borate buffered saline(20 mmol/L,pH 9.3),separation voltage of 20 KV by pressure injection(injection pressure was 50 bar with 3 s), detection wavelength was 350 nm,and column temperature was 30 ℃. RESULTS:The linear range of calceolarioside A was 50-250μg/ml(r=0.999 7);RSDs of precision,stability and reproducibility tests were less than 3%,recovery was 97.48%-102.08%(RSD=1.12,n=6). CONCLUSIONS:The method is simple,good reproducibility,and can be used for the content determination of calceolarioside A in the Leaves of F. mandschurica.
