Effects of HO-1 on Lipopolysaccharide-induced Endoplasmic Reticulum Stress of Rat Hepatocytes
10.3969/j.issn.1004-5619.2015.06.001
- VernacularTitle:HO－1对脂多糖诱导大鼠肝细胞内质网应激的作用
- Author:
Yansha WANG
;
Yinglei JI
;
Tao WANG
;
Linlin WU
;
Chengping FEI
;
Yichang LIU
;
Zhenyong GU
- Publication Type:Journal Article
- Keywords:
forensic pathology;
RNA,sm all interfering;
endoplasm ic reticulum;
stress;
lipopolysaccha-rides;
hem e oxygenase-1;
hepatocytes;
rats
- From:
Journal of Forensic Medicine
2015;(6):417-421
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of antioxidant stress protein hem e oxygenase-1 (HO-1) on lipopolysaccharide (LPS)-induced endoplasm ic reticulum stress (ERS) of rat hepatocytes. Methods The BRL cells (rat hepatocyte cell line) were cultured. The hepatocytes were treated with LPS, LPS+HO-1 si RNA , HO-1 siRNA and PB S solution, respectively. The cell viability was m easured by trypan blue ex-clusion test. The apoptosis cells were detected by the fluorescent dye Hoechst 33258. E xpressions of GR P78, C HO P, caspase-12 and HO-1 were detected by Western blotting. Results LPS caused an in-crease of HO-1 protein expression of rat hepatocytes in a dose-dependent and tim e-dependent m anner, a up-regulation of GRP78, CHO P and caspase-12, a decrease in cellviability,and an increase in apopto-sis rate of hepatocytes. Pretreatm ent of HO-1 siRNA inhibited the up-regulation of LPS-induced HO-1, however, aggravated ERS and cellular injury. Conclusion HO-1 inhibites ERS-m ediated cellular injury of rat hepatocytes induced by LPS.
