Isolation and Purification of Isoaloeresin D and Aloin from Aloe vera by High-speed Counter-current Chromatography
10.3969/j.issn.1674-6384.2010.02.010
- Author:
Jinzhi WAN
;
Xinxia CHEN
;
Chunmei QIU
;
Xiangcheng LIN
- Publication Type:Journal Article
- Keywords:
Aloe vera;
aloin;
high-speed counter-current chromatography;
isoaloeresin D;
extraction and purification
- From:
Chinese Herbal Medicines
2010;02(2):148-152
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an efficient method to isolate and purify the main components isoaloeresin D and aloin from Aloe vera for its industrial production. Methods High-speed counter-current chromatography was used to isolate isoaloeresin D and aloin in a one-step separation from dried crude extract ofA. vera. The biphasic solvent system the lipophilic phase was selected as the mobile phase and the apparatus was rotated at 840 r/min. The effluent was detected at 254 nm. Results Isoaloeresin D (53.1 mg) and aloin (106.9 mg) were separated from the crude extract (384.7 mg) with the purities of 98.6% and 99.5%, respectively. Conclusion HSCCC is a powerful technique for isolation and separation of chemical composition from aloe.
