Effect of n-butanol Extract from Potentilla anserina on Hypoxiainduced Calcium Overload and SERCA2 Expression of Rat Cardiomyocytes
10.3969/j.issn.1674-6384.2012.02.008
- Author:
Lingzhi LI
;
Lujun WANG
;
Yue WANG
;
Ying CUI
;
Jianyu LI
;
Li ZHANG
;
Yongliang ZHANG
- Publication Type:Journal Article
- Keywords:
cardiomyocyte;
hypoxia;
intracellular Ca2+;
Potentilla anserina;
sarcoplasmic reticulum
- From:
Chinese Herbal Medicines
2012;04(2):142-149
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of n-butanol extract from Potentilla anserina (NP) intervention on hypoxia-induced Ca2+ overload and SERCA2 expression of rat cardiomyocytes.MethodsPrimary cultured myocardial cell from SD neonatal rat (1-3 d) was used in the establishment of hypoxia model.After hypoxia for 3 h,the Ca2+ concentration of myocardial cells was measured with fura-2/AM fluorescent probe,and the biochemical indicator intracellular Ca2+-ATPase was examined and the mRNA and its protective protein levels of the sarcoplasmic reticulum (SR) Ca2+-ATPases (SERCA2) were assayed with RT-PCR,Western-blotting,and immune-cytochemical staining in each group.ResultsThe results showed that NP decreased Ca2+ concentration,increased the activity of Ca2+-ATPase,and improved the mRNA and protein expression of SERCA2 in hypoxia-injured myocardial cells as compared with the model group.ConclusionThese results indicate that NP could attenuate the Ca2+ overload.The mechanism might be explained as that NP could elevate the SERCA2 level,increase the activity of myocardium in rats,and further enhance the capacity of SR Ca2+ re-uptake.
