Effect of cIAP1 down-regulation mediated by shRNA on biological behavior of human ovarian cancer cells

VernacularTitle:cIAP1基因的 RNA 干扰载体的构建及其对卵巢癌细胞的生物学影响
Author: Hong JIN ; Kai XIE ; Meili ZHANG ; Jing SUN ; Youyuan DONG
Publication Type:Journal Article
Keywords: Cellular inhibitor of apoptosis protein 1 ( cIAP1 ); Ovarian cancer; RNA interference (RNAi)
From: Practical Oncology Journal 2015;(1):17-21
CountryChina
Language:Chinese
Abstract: [Abstratc] Objective The function of cIAP1 in the progression of ovarian cancer has not been clarified . This study is to explore the involvement of cIAP 1 in regulating biological behaviors of ovarian cancer cells by u-sing RNA interference(RNAi)technology.Mte hods The short hairpin RNA plasmid targeting cIAP1 was con-structed and transfected into Skov 3 cells.The levels of cIAP1 mRNA and protein were investigated by RT -PCR and Western Blot respectively .MTT assay and flow cytometry were used to evaluate cell proliferation and apopto-sis.R esults The rate of cIAP1 transfection was 74.7%performed by flow cytometric analysis .cIAP1 expression was significantly down -regulated at both mRNA and protein levels ,which resulted in a decrease of cell prolifera-tion and invasion capability in vitro .Conclusion This study implies that cIAP 1 might play an important role in the progression of ovarian cancer ,and it could be a potential target for therapeutic anti -cancer drugs .