Construction and expression of HSV1-tk eukaryotic vector in lung adenocarcinoma AGZY cell line
10.3969/j.issn.1002-3070.2014.02.001
- VernacularTitle:HSV1-tk 报告基因真核表达载体的构建及其在人肺腺癌 AGZY 细胞中的表达
- Author:
Sha LUAN
;
Peng FU
;
Zhongnan JIN
;
Guomei TIAN
;
Tingjun JIANG
;
Xueliang CAO
;
Changjiu ZHAO
- Publication Type:Journal Article
- Keywords:
Herpes simplex virus type 1 thymidine kinase;
Eukaryotic expression vector;
Reporter gene imaging;
Lung adenocarcinoma AGZY cell
- From:
Practical Oncology Journal
2014;(2):97-101
- CountryChina
- Language:Chinese
-
Abstract:
Objective The purpose of this study is to construct eukaryotic gene vector of herpes simplex virus type 1 thymidine kinase(HSV1-tk)and to observe the expression of HSV1-tk in lung adenocarcinoma AGZY cell line.Methods The full length HSV1-tk gene was amplified by PCR from plasmid pHSV 106 and was inserted into pMD18-T.The recombinant plasmid was recombined with eukaryotic vector plRES 2-EGFP u-sing gene recombinant technique .HSV1 -tk was transfected into adenocarcinoma AGZY cell line with Lipo-fectamineTM 2 000.Fluorescence microscopy was used to detect the transfection and expression of HSV 1-tk.RT-PCR was used to detect the mRNA levels of HSV 1-tk.The cell proliferation was measured by MTT assay .Re-sults A length of 1 130 bp gene sequence was obtained by PCR .The expressions of HSV 1-tk at mRNA and protein levels were displayed by RT -PCR and Western blot .MTT analysis showed that there were no significant changes cell survival on after transfection .Conclusion The eukaryotic expression vector of HSV 1 -tk report gene is successfully constructed and HSV 1-tk is effectively expressed in transfected AGZY cells .
