Fourier Transform Infrared Microspectroscopy of Rat Kidney with Regard to Fa-tal Hyperthermia

VernacularTitle:高温环境下死亡大鼠肾傅里叶显微红外光谱变化
Author: Zhijun WANG ; Shanshan SHEN ; Kaifei DENG ; Zhiqiang QIN ; Ping HUANG ; Zhenyuan WANG
Publication Type:Journal Article
Keywords: forensic pathology; spectroscopy,Fourier transform infrared; cause of death; hypertherm ia; kidney; rats
From: Journal of Forensic Medicine 2015;(4):257-261
CountryChina
Language:Chinese
Abstract: Objective T o observe the chem ical groups changing in rat kidney w ith regard to fatal hyper-therm ia by Fourier transform infrared m icrospectroscopy (FT IR-M SP ) and to provide a new m ethod to diagnose fatal hypertherm ia. Methods R ats w ere sacrificed by hypertherm ia, brainstem injury, m assive hem orrhage and asphyxiation and divided into groups. T he renal sam ples w ere dissected im m ediately af-ter death. T he data of infrared spectroscopy in glom erulus w ere m easured by FT IR-M SP. Results T he absorbances of 3 290, 3 070, 2 850, 1 540 and 1 396 cm -1 significantly increased (P<0.05),and the ratios of A1650/A3290 and A1650/A1540 significantly decreased (P<0.05) in group of hypertherm ia. Conclusion FTIR-M SP can analyze the changes of chem ical groups of kidney as an auxiliary diagnosis for discrim inating hyper-therm ia w ith other causes of death.