Validation of GlobalFiler?PCR Amplification Kit and the STR Polymorphism
10.3969/j.issn.1004-5619.2015.04.005
- VernacularTitle:GlobalFiler?PCR扩增试剂盒验证及其STR遗传多态性
- Author:
Zhiyong LU
;
Luyan XUE
;
Qingxia ZHANG
;
Yi ZHAO
;
Jinjie LIU
;
Hui TANG
- Publication Type:Journal Article
- Keywords:
forensic genetics;
polym orphism,genetic;
short tandem repeat;
G lobalFiler?PCR Amplifica-tion Kit
- From:
Journal of Forensic Medicine
2015;(4):273-276
- CountryChina
- Language:Chinese
-
Abstract:
Objective To test the technical param eters of GlobalFiler?PC R A m plification K it for its ap-plication to forensic application value and to investigate the genetic polym orphism s. Methods The valida-tion w as conducted in sensitivity, m ixed sam ples, species specificity, adaptability, survivability, consistency, peak height balance and stability. The am plification and detection of the genom ic D N A from 373 unre-lated individuals from B eijing H an nationality w ere extracted by autom ation w orkstation. Results Global-Filer?PC R A m plification K it w as adaptive to som e m ixed, degraded and inhibited sam ples. The pow er of sensitivity and adaptability and peak height balance show ed w ell. The distributions of genotype fre-quencies for 21 STR loci in the population w ere all in accordance w ith H ardy-W einberg equilibrium (P>0.05). The PIC value of the 21 STR loci w as am ong 0.536 to 0.940; the H value w as am ong 0.558 to 0.933; the D P value w as am ong 0.783 to 0.992; the PE value w as am ong 0.243 to 0.874. Conclusion GlobalFiler?PC R A m plification K it is suitable for crim inal cases and D N A database in forensic practice. A nd 21 STR loci in B eijing H an nationality have high polym orphism , w hich have ap-plication value in forensic practice and population genetics.
