Research of roles of NF-κB and promote apoptosis protein Bak in ucelrative coli-tis
10.3969/j.issn.1000-484X.2016.09.009
- VernacularTitle:NF-κB介导的促凋亡蛋白Bak表达在溃疡性结肠炎发病中的作用研究
- Author:
Xiao CHEN
;
Qizhi WANG
;
Yiwen LI
;
Wenjing MA
;
Donghong YU
- Publication Type:Journal Article
- Keywords:
NF-κB;
Bak;
Ucelrative colitis
- From:
Chinese Journal of Immunology
2016;32(9):1286-1290
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression levels of nuclear factor kappa B, Bcl-2 associated K and TNF-αproteins to discuss the effects of NF-κB and Bak proteins in the pathogenesis of UC.Methods:Eighty clean grade of adult Sprague-Dawley( SD) rats were used,male and female in half and then rando mly selected sixty as the model group,another twenty as the control group.SD rats model were manufactured by a compound method:Trinitrobenzene sulfonic acid ( TNBS )+ethanol.We observed and assessed colonic mucosa by the general morphology and histological changes.To applicated immunohistochemistry and RT-PCR methods to detected the protein and mRNA expression levels of NF-κB,Bak and TNF-αin the model groups and the control group and to analysed their relationships.Results:The successful rate of making model was 97%.The number of inflammatory cells in the model groups more than the control(P<0.01).Group immunohistochemical and RT-PCR,NF-κB and TNF-αproteins and mRNA in UC colon epithelium cells and inflammatory cells were higher than the control(P<0.01).Bak protein in inflammatory cells were lower than the control(P<0.01),but there was no statistical significance in epithelial cells(P>0.05).The expression levels of NF-κB,TNF-αincreased as the histological grade increased(P<0.05),however,the expression level of Bak decreased(P<0.05).NF-κB in colonic mucosa of rats with UC had a significantly positive correlation with that of TNF-α(r=0.892,P<0.01),and negatively correlated with that of Bak(r=-0.793,P<0.01).Conclusion:The levels of NF-κB and Bak may be related to the occurrence and development of UC.
