Effect of AGEs on expression of mitochondrial fusion proteins Mfn1 and Mfn2 in cultured human aortic endothelial cells
10.3969/j.issn.1000-4718.2016.09.026
- VernacularTitle:AGEs 对人主动脉内皮细胞线粒体融合蛋白表达的影响
- Author:
Shunrong ZHANG
;
Yue GAO
;
Fei FENG
- Publication Type:Journal Article
- Keywords:
Advanced glycation end products;
Human aortic endothelial cells;
Mitochondrial dynamics;
Mito-chondrial fusion proteins
- From:
Chinese Journal of Pathophysiology
2016;32(9):1688-1693
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To clarify whether advanced glycation end products (AGEs) can influence the expression of mitochondrial fusion proteins Mfn1 and Mfn2 in cultured human aortic endothelial cells (HAECs) in vitro.METHODS:AGE-BSA was used as AGEs.Purchased primary human aortic endothelial cell line was multiplied, and transferred to dif-ferent passages for subsequent grouping.For dose-dependent experiment, HAECs were divided into 4 groups, and the con-centrations of AGE-BSA in each group were 0 mg/L (control group), 50 mg/L, 100 mg/L and 200 mg/L, respectively. For time-dependent experiment, HAECs were divided into 5 groups with the same concentration (100 mg/L) of AGE-BSA, but the intervention time was 0 h (control group), 6 h, 12 h, 24 h and 48 h, respectively.The mRNA and protein expres-sion levels of Mfn1 and Mfn2 in the HAECs were detected by real-time PCR and Western blot, respectively.RESULTS:Exposure of the HAECs to AGEs at different concentrations for 24 h all down-regulated the mRNA and protein expression levels of Mfn1 and Mfn2.Except for 6 h intervention group, 100 mg/L AGEs intervention for 12 h, 24 h and 48 h all down-regulated the mRNA and protein expression levels of Mfn1 and Mfn2 in cultured HAECs.CONCLUSION: AGEs down-regulates the expression of mitochondrial fusion proteins Mfn1 and Mfn2 in cultured HAECs, indicating that AGEs may influence mitochondrial dynamics of human aortic endothelial cells.
