Effects of IL-13 on SDF-1 and EGF expression in fibroblasts co-cultured with breast cancer cells
10.3969/j.issn.1000-484X.2016.09.013
- VernacularTitle:白介素-13对与乳腺癌细胞共培养的成纤维细胞 SDF-1和 EGF 表达影响
- Author:
Xiaoyu SHI
;
Shaofen CHEN
;
Huaping GU
;
Hui LU
;
Wenlin LI
- Publication Type:Journal Article
- Keywords:
Interleukin-13;
Fibroblast;
Breast tumor;
SDF-1;
EGF
- From:
Chinese Journal of Immunology
2016;32(9):1303-1308
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The effects of IL-13 ( Interleukin-13 ) on SDF-1 ( Stromal cell derived factor 1 ) and EGF ( Epidermal growth factor) expression in fibroblasts co-cultured with breast cancer cells were investigated to explore the mechanism for IL-13 in the development of breast cancer.Methods:The co-culture of human breast cancer cell line MDA-MB-231 with the human skin fibroblast line CCC-ESF-1 ( ESF) was used in vitro and in tumor-burdened nude mice.The effects of IL-13 on SDF-1 and EGF expression in the co-cultured fibroblasts in vitro were analyzed using reverse transcription quantitative polymerase chain reaction ( RT-qPCR ) , flow cytometry and Western blot assay.The proliferation of the co-cultured human breast cancer cells in vitro was detected by Cell counting kit-8(CCK-8).The effects of IL-13 on SDF-1 and EGF expression in the fibroblasts of tumor tissue of tumor-burdened nude mice were analyzed using immunofluorescence and laser confocal microscope, and the tumor volumes were examined.Results: IL-13 could up-regulate SDF-1 and EGF expression in the fibroblasts co-cultured with breast cancer cells in vitro,and promoted the proliferation of the co-cultured breast cancer cells.In tumor-burdened nude mice,IL-13 enhanced SDF-1 and EGF expression of fibroblasts in tumor tissue, and accelerated tumor growth.Conclusion:IL-13 up-regulates SDF-1 and EGF expression of fibroblasts co-cultured with breast cancer cells.The molecular mechanism of promoting effect of IL-13 on breast cancer relates to SDF-1 and EGF of fibroblasts in breast cancer stroma.
