The feasibility study of radiation absorbed dose measurement using oligonucleotide dually labeled with Alexa Fluor 488 and BHQ1 probe
10.3760/cma.j.issn.0254-5098.2016.06.004
- VernacularTitle:应用Alexa Fluor 488与BHQ1双标记寡核苷酸荧光探针测量辐射吸收剂量的可行性研究
- Author:
Wenwen LIN
;
Panying SHI
;
Baoguo ZHANG
- Publication Type:Journal Article
- Keywords:
Fluorescent probe;
Alexa Fluor 488;
BHQ1
- From:
Chinese Journal of Radiological Medicine and Protection
2016;36(6):419-423
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the feasibility of measuring radiation absorbed dose with the fluorescent probe Alexa Fluor 488-DNA-BHQ1.Methods An oligonucleotide dually labeled at its 5'-and 3'-end with fluorescent molecular Alexa Fluor 488 and specific fluorescence inhibitors BHQ1 was prepared.The Alexa Fluor 488-DNA-BHQ1 aqueous solution was exposed with X-ray and its fluorescence intensity was measured.Results When the concentration of Alexa Fluor 488-DNA-BHQ1 was between 0.5 and 1 μmol/L,the fluorescence intensity of its aqueous solution had excellent linear dose response from 0.1 to 30 Gy (R2 =0.99) and it was stably maintained after 40-80 min of irradiation especially at 4℃.Conclusions In the dose range of 0.1-30 Gy,the Alexa Fluor 488-DNA-BHQ1 fluorescent probe can be used to measure radiation absorbed dose.
