Immuno-regulatory effects on murine macrophages by low dose naltrexone (LDW)

VernacularTitle:低剂量纳曲酮对小鼠巨噬细胞的免疫调节作用及机理研究
Author: Shengnan GUO ; Yan LI ; Xiaonan WANG ; Fengping SHAN
Publication Type:Journal Article
Keywords: Naltrexone; Macrophage; Phenotype; Cytokine; Phagocytosis
From: Chinese Journal of Immunology 2016;32(7):970-973
CountryChina
Language:Chinese
Abstract: Objective:The effect of naltrexone on function of murine peritoneal macrophage in vitro was studied in order to illuminate its immune activity futher. Methods:Peritoneal macrophages were divided in three groups:RPMI1640 blank control group, LPS positive control group and NTX treated group. Various phenotypic and functional indices were tested by MTS, flow cytometry technology,phagocytosis experiment and ELISA. Results: Compared with RPMI1640 group,at a LDN exhibits paradoxical properties;the expression of CD64 on surface increased while the expression of CD206 decreased in NTX group;the expression of tumour necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-1β(IL-1β)were increased. Conclusion:The results of experiment had proved that LDN could influence macrophage polarzation, regulate the inflamatory mediators production and affect the phagocytosis function of peritoneal macrophage.