miR-222 enhances HBx-HepG2 cell growth via regulation of BCL2L13 gene
10.3969/j.issn.1000-4718.2016.08.008
- VernacularTitle:miR－222通过调控 BCL2 L13基因促进 HBx－HepG2细胞生长
- Author:
Guifang YU
;
Shudi CHEN
;
Xuezhu CHEN
;
Kailian HOU
;
Min LIANG
- Publication Type:Journal Article
- Keywords:
MicroRNA-222;
HBx-HepG2 cells;
Cell growth;
Cell cycle;
Apoptosis;
BCL2L13 gene
- From:
Chinese Journal of Pathophysiology
2016;32(8):1389-1394
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the regulation of miR-222 on BCL2L13 gene and its effect on the growth and apoptosis of HBx-HepG2 cells, and to explore the underlying molecular mechanisms .METHODS:The expression level of miR-222 was detected by RT-qPCR.The HBx-HepG2 cell growth was examined by MTT and colony formation assays .The cell cycle and apoptosis were analyzed by flow cytometry .The recombination vector pmirGLO-BCL2L13 was constructed, and dual-luciferase reporter experiment was performed to validate the target of miR-222.RESULTS:The expression level of miR-222 in the HBx-HepG2 cells was significantly higher than that in the L 02 cells ( P<0.05 ) .Over-expression of miR-222 enhanced HBx-HepG2 cell growth, changed cell cycle, and inhibited apoptosis (P<0.05).Knockdown of miR-222 reduced HBx-HepG2 cell growth, changed cell cycle, and increased cell apoptotic rate (P<0.05).BCL2L13 was down-regulated in the HBx-HepG2 cells as compared with L02 cells (P<0.05), and knockdown of miR-222 in the HBx-HepG2 cells increased the expression level of BCL2L13 (P<0.05).The results of dual-luciferase reporter assay and re-store experiment showed that miR-222 negatively regulated the expression of BCL2L13 via targeting 3’ UTR of BCL2L13, resulting in the promotion of HBx-HepG2 cell growth .CONCLUSION: miR-222 enhances HBx-HepG2 cell growth via down-regulation of BCL2L13.
