The changes of regulatory B cell subset in children with type 1 diabetes mellilus

VernacularTitle:1型糖尿病患儿调节性B细胞变化初探
Author: Shilei JIA ; Chengrong LI ; Jun YANG ; Guobing WANG ; Xia LIU ; Weibin LI
Publication Type:Journal Article
Keywords: Diabetes mellitus,type 1; Regulatory B cells; Regulatory T cells; Interluekin-10
From: Chinese Journal of Endocrinology and Metabolism 2016;32(7):584-586
CountryChina
Language:Chinese
Abstract: [Summary] The proportions of CD19+CD24 hi CD38 hi regulatory B cells ( Breg ) and CD4+CD25+Treg in peripheral blood of children with type 1 diabetes mellitus ( T1DM) were detected by flow cytometric analysis. The concentration of interleukin 10 (IL-10) protein was measured by ELISA. The mRNA expression levels of Foxp3, cytotoxic T lymphocyte-associated antigen-4 ( CTLA-4 ) , and glucocorticoid-induced tumor necrosis factor receptor ( GITR) in CD4+T cells and IL-10 in CD19+ B cells were evaluated using real-time PCR. The results showed that the proportions of CD19+CD24hiCD38hiBreg in peripheral blood, the mRNA and protein levels of IL-10 in B cells from patients with T1DM were lower than those from healthy controls (P<0. 05). The mRNA expression levels of Treg associated factors such as Foxp3, CTLA-4, and GITR were lower in CD4+ T cells from children with T1DM compared with the controls(P<0. 05). These results suggest that Breg cell deficiency and dysfunction might be one of the important factors causing cellular immune dysfunction in patients with T1DM.