Effect of 5-Aza-CdR with MS275 on growth and expression of DNMT1 protein in cell lines of non-small cell lung cancer
10.3969/j.issn.1006-5725.2016.13.009
- VernacularTitle:5-氮杂-2′-脱氧胞苷和MS-275对非小细胞肺癌细胞株生长及DNMT1蛋白表达的影响
- Author:
Bangqing LIU
;
Jiaqiang LUAN
;
Xiaolin SUN
;
Jian BAI
;
Jianfei SONG
;
Zhenzong DU
- Publication Type:Journal Article
- Keywords:
Non-small cell lung cancer;
Epigenetic treatment;
DNA methyltransferase 1;
DNA methylation
- From:
The Journal of Practical Medicine
2016;32(13):2105-2108
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the characteristics and biological functions of epigenetic treatment to DNMT1 expression in NSCLC cell lines. Methods Lung cancer cells were divided into 4 groups , and drugs with different concentrations. The effect of epigenetic treatment on NSCLC cell line was detected by CCK-8 and MTT. The effect of epigenetic treatment on the expression of DNMT1 in NSCLC cell line was detected by Western blot. Results CCK-8 and MTT assay showed that treatment with 5-Aza-CdR and MS-275 inhibited the proliferation of NSCLC cells. Western blot showed that treatment with 5-Aza-CdR and MS-275 inhibited the DNMT1 expression in NSCLC cells. Conclusion The expression of DNMT1 gene in the NSCLC cell lines may be suppressed effectively by epigenetic treatment , and the gene may inhibit the proliferation and growth of NSCLC cell lines.
