Investigation for anti-tumor effects of CIK cells induced by TLR7 agonist instead of IFN-γ
10.3969/j.issn.1000-484X.2016.08.016
- VernacularTitle:TLR7激动剂替代IFN－γ诱导CIK细胞杀伤肿瘤的研究
- Author:
Wang LI
;
Yanyuan CHEN
;
Zhizhang WEI
;
Yuhuan WANG
;
Xiaoling LUO
;
Dong GAO
- Publication Type:Journal Article
- Keywords:
Immune cells;
Toll like receptor 7 agonist;
Antitumor;
Leukemia
- From:
Chinese Journal of Immunology
2016;32(8):1157-1159
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the immune effects of CIK cells induced by Toll like receptor 7 agonist (Tlr7a) instead of IFN-γon killing lymphoma cells in vitro .Methods: Mononuclear cells were isolated from healthy human peripheral blood .CIK were induced by Tlr7a in vitro instead of IFN-γ.Two groups were divided as follows:CIK group,Tlr7a-CIK group.Then the main investigation on immune effects included immune phenotype was detected respectively , and cytotoxicity of the effectors was analyzed .Results: In Tlr7a-CIK group,the amount of CD56+cells was more than CIK group (P<0.05),and the cytotoxicity was also stronger (P<0.05). Conclusion:Tlr7a instead of IFN-γcould promote the immune effects of CIK cells on killing tumor cells in vitro .
