Effect and its significance of Wnt/β-catenin signaling pathway by controlling tumor marker CD44 on nasopharyngeal carcinoma SP cells
10.16066/j.1672-7002.2016.04.007
- VernacularTitle:Wnt/β-catenin信号通路调控下游基因CD44对鼻咽癌侧群细胞的作用和意义
- Author:
Shanshan XIONG
;
Qianhui QIU
;
Xiaoning LUO
;
Yong CUI
;
Xiaomei SU
;
Mimi XU
- Publication Type:Journal Article
- Keywords:
Nasopharyngeal Neoplasms;
Tumor Stem Cell;
Flow Cytometry;
Cell Cycle;
wnt/β-catenin signaling pathway;
CD44;
tumor marker
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2016;23(4):211-215
- CountryChina
- Language:Chinese
-
Abstract:
[ABSTRACT]OBJECTIVEThis study aims to explore the molecular mechanism and expression of Wnt/β-catenin signaling pathway and tumor marker CD44 in nasopharyngeal carcinoma cells after transfection withβ-Catenin when the Wnt/β-catenin signaling pathway was blocked.METHODSSP cells and CD44+SP cells isolated from the nasopharyngeal carcinoma cell line CNE-2 by flow cytometry were identified. Changes in the number and biological characteristics of CNE-2 and CD44+SP cells in vitro were investigated after the Wnt/β-catenin signaling pathway was blocked through siRNA.RESULTSSP cells accounted for 2.3% of nasopharyngeal carcinoma CNE-2 cells, andCD44+SP cells accounted for 36.5% of the SP cells. CD44+SP cells showed significantly higher in vitro proliferation and resistance to chemotherapy (P<0.05). After transfection withβ-Catenin siRNA, the proliferation, cloning efficiency, and tolerance to chemotherapeutic drugs of the cells were found statistical differences compared with those before transfection ofβ-Catenin siRNA. CONCLUSIONWnt/β-catenin signaling pathway abnormalities are closely related to the biological behavior of nasopharyngeal carcinomaCD44+SP cells. Interference of this pathway can change the characteristics of nasopharyngeal carcinoma stem cells.
