Uncertainty Evaluation of the Determination of Lamotrigine in Human Plasma by LC-MS/MS
- VernacularTitle:LC-MS/MS 法测定人血浆中拉莫三嗪浓度的不确定度评定
- Author:
Li YANG
;
Leting ZHU
;
Weixing FENG
;
Zhigang ZHAO
;
Shenghui MEI
- Publication Type:Journal Article
- Keywords:
LC-MS/ MS;
Lamotrigine;
Human plasma;
Uncertainty
- From:
China Pharmacist
2016;19(5):854-858
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the uncertainty in the determination of lamotrigine(LTG)in human plasma by LC-MS/ MS. Methods:The uncertainty sources in the determination of LTG were analyzed and the uncertainty was evaluated and combined. Re-sults:The expanded uncertainty of LTG at low(0. 050 4 μg· ml - 1 )and high(1. 27 μg· ml - 1 )concentrations was 0. 005 18 μg· ml - 1 and 0. 066 4 μg· ml - 1 ,respectively(P = 95% ,k = 2). Conclusion:The uncertainty in the determination of LTG in human plasma by LC-MS/ MS is mainly caused by the protein precipitation recovery,matrix effect and sample preparation at low concentration, and by the matrix effect,sample preparation and repeatability at high concentration.
