Effects of combined administration of Ephedrae-Cinnamomi on pharmacokinetics of ephedra alkaloids,cinnamic acid and cinnamic alcohol in rats
10.3969/j.issn.1001-1978.2016.06.026
- VernacularTitle:麻黄－桂枝配伍对麻黄类生物碱、桂皮酸及桂皮醇在大鼠体内药动学的影响
- Author:
Ping WEI
;
Feilong CHEN
;
Qinhai MA
;
Mengyue REN
;
Jiabo LUO
- Publication Type:Journal Article
- Keywords:
Ephedrae;
Cinnamomi;
Ephedrae-Cinna-momi herb couple;
ephedra alkaloids;
cinnamic acid;
cinnamic alcohol;
plasma pharmacokinetics;
UPLC-MS/MS
- From:
Chinese Pharmacological Bulletin
2016;32(6):873-880
- CountryChina
- Language:Chinese
-
Abstract:
Aims Tocomparethepharmacokineticsof ephedra alkaloids,cinnamic acid and cinnamic alcohol in Ephedrae, Cinnamomi and Ephedrae-Cinnamomi herb couple through UPLC-MS/MS in rats respective-ly,and to investigate the effect of combination on phys-iologicaldisposition.Methods Plasmasampleswere collected at different times after oral administration of Ephedrae,Cinnamomi and Ephedrae-Cinnamomi herb couple extracts.The concentrations of ephedra alka-loids,cinnamic acid and cinnamic alcohol in plasma samples were determined by UPLC-MS/MS.DAS 3. 0 was used to calculate pharmacokinetic parameters.The differences of samples in two groups were conducted with univariate statistical analysis using SPSS 13. 0. Results ComparedwithEphedraegroup,theCmaxof norephedrine hydrochloride, norpseudoephedrine hydrochloride,ephedrine hydrochloride,pseudoephed-rine hydrochloride,and methylephedrine hydrochloride in Ephedrae-Cinnamomi herb couple group were signif-icantly greater (P<0. 05 );the AUC0-t of norpseudo-ephedrine hydrochloride was significantly greater (P<0. 05 );the MRT0-t of norephedrine hydrochloride, phedrine hydrochloride, pseudoephedrine hydrochlo-ride,and methylephedrine hydrochloride were signifi-cantly less (P <0. 05 );the T1/2z of norephedrine hydrochloride,phedrine hydrochloride,and methyl-ephedrine hydrochloride were significantly less (P <0. 05 ).The AUC0-t and MRT0-t of cinnamic acid and cinnamic alcohol were significantly greater than those inCinnamomigroup(P<0.05).Conclusion The combination of Ephedrae and Cinnamomi improves the absorption concentration of five ephedra alkaloids, Slows down the elimination of norephedrine hydrochlo-ride,phedrine hydrochloride,pseudoephedrine hydro-chloride,and methylephedrine hydrochloride,and in-creases the bioavailability of cinnamic acid and cin-namic alcohol.
