Effects of fibroblast growth factor inducible 14 (Fn14) on high glucose induced-cardiomyocyte hypertrophy
10.3760/cma.j.issn.1000-6699.2016.05.012
- VernacularTitle:Fn14在高糖诱导心肌细胞肥厚中的作用及机制
- Author:
Jiefang ZHANG
;
Zhida SHEN
;
Lu YU
;
Wenbing JIANG
;
Zhengwei LI
;
Guosheng FU
- Publication Type:Journal Article
- Keywords:
Fn14;
Cardiomyocyte hypertrophy;
JNK signaling pathway
- From:
Chinese Journal of Endocrinology and Metabolism
2016;32(5):411-414
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanisms of factor fibroblast growth factor inducible 14(Fn14)in the high glucose induced-cardiomyocyte hypertrophy. Method To observe the expression of collagenⅠ, connective tissue growth factor ( CTGF ) , transforming growth factor-β1 ( TGF-β1 ) , and Fn14 in high glucose induced-cardiomyocyte hypertrophy. Fn14 expressions was down-regulated by siRNA interference technique, and then the expressions of collagen Ⅰ, CTGF, and TGF-β1 were observed, and the mechanism was also explored. Results The expression of collagen I, CTGF and TGF-β1 was significantly up-regulated after high glucose induced-cardiomyocyte hypertrophy for 72 h. At the same time, the expression of Fn14 was increased after 72 h-treatment, and reached the peak at concentration of 30 mmol/L high glucose. High glucose could not up-regulated the expression of collagenⅠ, CTGF, and TGF-β1 after siFn14 interference, while the same result was observed in the expression of p-JNK. Conclusion The expressions of collagenⅠ, CTGF, TGF-β1, and Fn14 in cardiomyocyte of neonatal rats were induced by high glucose. While Fn14 expression was inhibited, the expressions of collagenⅠ, CTGF, and TGF-β1 were down-regulated, which seems to be involved with p-JNK signaling pathway.
