Study on melatonin in suppression osteoblast proliferation and reducing the ratio of OPG/RANKL expression
10.3760/cma.j.issn.1008-6706.2016.15.001
- VernacularTitle:褪黑素抑制成骨细胞的增殖并降低OPG／RANKL 表达比值的研究
- Author:
Peng JIANG
;
Jie JIANG
;
Jiabo WANG
;
Chao GAO
;
Haibo FENG
;
Yedong CHENG
- Publication Type:Journal Article
- Keywords:
Osteoblast;
Osteoporosis;
Melatonin
- From:
Chinese Journal of Primary Medicine and Pharmacy
2016;23(15):2241-2243
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of melatonin on osteoblast proliferation and osteogenic poten-tial in vitro cultured.Methods In vitro cultured human osteoblasts by different concentrations of melatonin (0, 1.0E -7,1.0E -6,1.0E -5,1.0E -4,1.0E -3mol/L)intervention 24 h,48 h,72 h,96 h.MTT assay was meas-ured by changes in cell proliferation,intervention in 96 hours,melatonin was measured by RT -PCR in cells OPG, RANK,RANKL influence,PTHLH mRNA expression.Results Compared with other groups,OD490 of the control group had statistical significance(all P <0.05).Compared with the control group,there were statistically significant differences in the ratio of OPG/RANKL of the dosing groups(all P <0.05).Conclusion Melatonin can promote the growth of bone into the cells and inhibit the action of bone cells into bone.
