Expression of bFGF and TGF-β1 in different stages of myocardial ifbrosis

VernacularTitle:碱性成纤维细胞生长因子及转化生长因子β1在心肌纤维化各时期的表达
Author: Tailing LU ; Ming LU ; Yuyin CHANG
Publication Type:Journal Article
Keywords: basic ifbroblast growth factor; transforming growth factor-β1; myocardial ifbrosis; rat
From: Journal of Clinical Pediatrics 2016;34(5):371-374
CountryChina
Language:Chinese
Abstract: Objective To study the expression of basic ifbroblast grouth factor (bFGF) and transforming growth factorβ1 (TGF-β1) in different stages of myocardial ifbrosis (CFs). Methods CFs of neonatal Sprague-Dawley rats were isolated with the method of trypsin digestion and differential anchoring velocity, then cultured in vitro. The generation 2-4 of CFs were used for the experiment and randomly divided into 2 groups:the control group were cultured without AngII , and the test group were cultured with AngII 10-6 mol/L. The test group were cultured for 12, 24, 48, and 72 h respectively, and then the synthesis of col agen were measured by ELISA, the bFGF, TGF-β1-mRNA expression was measured by RT-PCR, and the bFGF and TGF-β1 protein expression was measured by western blot analyses. Results Compared with those of control group, the expressions of bFGF and TGF-β1 both in gene and in protein in the test groups increased gradual y with the timing (P?