Clinical significance of expression of proliferating cell nuclear antigen and 8-hydroxydeoxyguanosine in gastric carcinoma
10.3969/j.issn.1006-5725.2016.08.022
- VernacularTitle:PCNA、8-OHdG在胃癌组织中的表达及意义
- Author:
Zhao PENG
;
Cuishi TIAN
;
Guisheng WANG
;
Huibin GAO
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Proliferating cell nuclear antigen;
8-hydroxydeoxyguanosine;
Im-munohistochemistry;
High-performance liquid chromatography
- From:
The Journal of Practical Medicine
2016;32(8):1274-1276
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of proliferating cell nuclear antigen ( PCNA) and the level of 8-hydroxydeoxyguanosine (8-OhdG) in gastric carcinoma and their clinical significance. Methods PCNA protein expression of 63 tumor tissues , 36 paracancerous tissues and 9 chronic gastritis mucosa were ana-lyzed by immunohistochemistry and 8-O HdG of hydrolysates was determined by HPLC. Results The expression of PCNA and the level of 8-OHdG were upregulated in chronic gastrits mucosa adjacent tissues and gastric carci-noma raised gradually and these two metrics were associated with tumor differentiation and TNM stage. Conclu-sions Up-regulation of PCNA protein expression and 8-O HdG levels are observed in gastric carcinoma. Detec-tion of PCNA protein expression and 8-O HdG levels can be used to evaluate malignant biological behavior and prognosis of gastric cancer.
