miRNA-126 inhibits proliferation, migration and invasion of human lung cancer A549 cells via EGFR/AKT/mTOR pathway
10.3969/j.issn.1000-4718.2016.03.012
- VernacularTitle:miRNA-126对肺癌 A549细胞的增殖、迁移、侵袭及 EGFR／AKT／mTOR 信号通路的影响
- Author:
Xiali TANG
;
Demin JIAO
;
Jun CHEN
;
Jian WANG
;
Qingyong CHEN
- Publication Type:Journal Article
- Keywords:
MicroRNA-126;
EGFR/AKT/mTOR pathway;
Lung cancer;
Cell proliferation
- From:
Chinese Journal of Pathophysiology
2016;32(3):458-463
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of microRNA ( miRNA)-126 on the proliferation , migration and in-vasion of human lung cancer cell lines , and to explore its mechanism .METHODS:The A549 cells were transfected with miRNA-126 agomir by Lipofectamine 2000.The expression of miRNA-126 was detected by real-time PCR.The cell activity was detected by MTT assay .The number of viable A549 cells was counted by the method of Trypan blue exclusion .The cell colony-forming capability was determined by cell colony formation test .The cell migration and invasion abilities were assayed by wound healing and Transwell methods , respectively.The protein levels of p-EGFR, EGFR, p-AKT, AKT, p-mTOR and mTOR were determined by Western blot .RESULTS:The expression level of miRNA-126 was significantly in-creased in the A549 cells compared with negative control ( NC) group and control group ( P<0.01 ) .The proliferation of A549 cells was decreased extremely after transfected with the miRNA-126 agomir (P<0.01), so did the result of the cell colony-formation test.The migration and invasion abilities of the lung cancer cells were also significantly inhibited .The protein levels of p-EGFR, p-AKT and p-mTOR were significantly down-regulated compared with NC group and control group ( P<0.01) .CONCLUSION:Over-expression of miRNA-126 significantly inhibits the proliferation , migration and invasion ability of human lung cancer A 549 cells by down-regulation of EGFR/AKT/mTOR pathway .
