Expression of TLR2 and TLR4 on mast cells in human chronic periapical diseases
10.3969/j.issn.1000-4718.2016.03.021
- VernacularTitle:TLR2和 TLR4在人慢性根尖周病组织肥大细胞上的表达
- Author:
Zixi TAO
;
Wanting FAN
;
Juan LI
;
Shiguang HUANG
- Publication Type:Journal Article
- Keywords:
Chronic periapical diseases;
Mast cells;
Toll-like receptor 2;
Toll-like receptor 4
- From:
Chinese Journal of Pathophysiology
2016;32(3):516-521
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the expression of TLR2 and TLR4 on mast cells (MCs) in the periapical tissues from different types of human chronic periapical diseases , and to analyze the role of TLR 2 and TLR4 on tryptase-positive MCs in the immunopathogenesis of human chronic periapical diseases .METHODS: A total of 60 donors, including healthy control group , periapical granuloma group and periapical cyst group , were enrolled in the study .The periapical tis-sue specimens were fixed in 10%buffered formalin and stained with hematoxylin and eosin for histopathology , or stained with double-immunofluorescence for identification of TLR 2-tryptase and TLR4-tryptase double-positive MCs in the periapical tissues.RESULTS:Compared with the healthy control , the densities of TLR2-tryptase and TLR4-tryptase double-positive MCs in periapical tissues were significantly increased in human chronic periapical diseases (P<0.01).The densities of TLR2-tryptase and TLR4-tryptase double-positive MCs in periapical cyst group were significantly higher than those in peria-pical granuloma group (P<0.01).CONCLUSION:TLR2 and TLR4 were expressed on the MCs in the periapical tissues of human chronic periapical diseases .TLR2-tryptase and TLR4-tryptase double-positive MCs may participate in the patho-genesis of chronic periapical diseases .
