Effect of Acupuncture plus Astragalus Polysaccharide on the Expression of Bcl-2 Protein in Islet ? Cells in db/db Mice
10.13460/j.issn.1005-0957.2016.06.0738
- VernacularTitle:针刺联合黄芪多糖对db/db小鼠胰岛β细胞Bcl-2蛋白表达的影响
- Author:
Wenkui ZHANG
;
Qian LI
;
Cuihong GONG
;
Zhi SUN
- Publication Type:Journal Article
- Keywords:
Acupuncture;
Astragalus polysaccharide;
Diabetes,non-insulin dependent;
βcell;
B-lymphocyte tumor protein;
Mice
- From:
Shanghai Journal of Acupuncture and Moxibustion
2016;35(6):738-741
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of acupuncture plus astragalus polysaccharide on the expression of Bcl-2 protein in pancreatic islet b cells in db/db mice. Method C57BL/Ksj-db/db mice as an animal model of spontaneous type 2 diabetes were selected for this experiment. Five-week-old db/db mice were randomized into model, acupuncture, medication and acupuncture+medication groups. Meanwhile, db/m mice were selected as a normal group. The acupuncture group received acupuncture at points Housanli (equivalent to Zusanli, ST36), Neiting(ST44) and Yishu(Extra) and the medication group, an oral gavage of astragalus polysaccharide (1400 mg/kg). Both groups were treated once daily, for 12 consecutive weeks. After the end of experiment, blood glucose, insulin and resistin were measured, and the expression of Bcl-2 protein in islet b cells was determined by immunohistochemical method. Result Blood glucose, insulin and resistin levels were significantly lower in the acupuncture+medication, acupuncture and medication groups than in the model group. They were significantly lower in the acupuncture+medication group than in the acupuncture and medication groups and significantly lower in the acupuncture group than in the medication group. The expression of Bcl-2 protein in islet b cells was higher in the medication, acupuncture and acupuncture+medication groups than in the model group; there was a statistically significant difference (P<0.01). The expression of Bcl-2 protein was higher in the acupuncture+medication groups than in the medication group (P<0.01) and it was basically the same in the medication group as in the acupuncture group; there was no statistically significant difference (P>0.05). Conclusion Acupuncture plus astragalus polysaccharide can significantly reduce blood glucose and serum insulin and resistin levels and increase the expression of Bcl-2 protein in islet b cells to effectively inhibit apoptosis in islet b cells in db/db mice. Its effect is better than that of acupuncture alone or medication.
