CO-029 mediates TNFα/TNFαR1 induced epithelial mesenchymal transition in intrahepatic cholangiocarcinoma

VernacularTitle:四跨膜蛋白CO-029介导肿瘤坏死因子诱导胆管癌上皮间质转化
Author: Liang CHEN ; Zhuori LI ; Kailun ZHOU ; Ming ZHAO ; Yehong WANG ; Jincai WU
Publication Type:Journal Article
Keywords: Intrahepatic cholangiocarcinoma; Transmembrane protein,CO-029; Tumor necrosis factor; Epithelial mesenchymal transition; Invasion; Metastasis
From: Chinese Journal of Hepatobiliary Surgery 2016;22(2):121-124
CountryChina
Language:Chinese
Abstract: Objective To investigate the role of transmembrane protein C0-029 in epithelial mesenchymal transition (EMT) induced by tumor necrosis factor alpha (TNFα) of intrahepatic cholangiocarcinoma (ICC) cells.Methods RT-PCR and Western blot were conducted to detect the CO-029 expression in ICC cells and tissues.The effect of CO-029 silencing by lenti-virus on EMT induced by TNFα was investigated.Western blot and mass spectrometry after immunoprecipitation were used to confirm whether TNFαR1 can directly or indirectly bind with CO-029 to form complexes in ICC.Results Differential expression of CO-029 was observed in ICC cells and tissues.The expression of CO-029 was significantly reduced by lenti-viral interference in ICC cells,resulting in the failure of TNFα to induce EMT.TNFαR1 in ICC could directly or indirectly form complexes with CO-029.Conclusion CO-029 mediates TNFα/TNFαR1 induced EMT in ICC,which might play an important role in the invasion and metastasis.