Effec ts of hypoxic preconditioning on learning and memory in mice with cerebral sichemia-reperfu-sion injury and underlyin g mechanisms
10.3760/cma.j.issn.1674-6554.2016.02.003
- VernacularTitle:低氧预适应对缺血再灌注脑损伤小鼠学习记忆的影响及机制研究
- Author:
Yuanyuan HUANG
;
Yanbo ZHANG
;
Meiyi LI
;
Mingfeng YANG
;
Tong ZHAO
;
Jingzhong NIU
- Publication Type:Journal Article
- Keywords:
Hypoxic preconditioning;
Ischemia-reperfusion;
Learning and memory;
Apopto-sis
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2016;(2):108-111
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of hypoxic preconditioning on learning and memory and the possible protective mechanism in mice with cerebral ischemia-reperfusion injury.Methods Healthy adult male Kunming mice were randomly divided into five groups by Random number table:normal group( N group),hypoxic preconditioning group (HPC group),sham operation group (C group),ischemia-reperfusion group(O group),hypoxic preconditioning and ischemia-reperfusion group(HPC+O group).HPC+O group were given hypoxic preconditioning before 24h of ischemia-reperfusion.The escape latency was detected by Morris water maze and the neuron apoptosis of CA 1 area of hippocampal was determined by immunofluores-cence techniqueR.e sults The escape latency in HPC+O group on the second,third and fourth day of MWM was (39.92±4.52)s,(30.98±2.44)s,(19.69±4.27)s,and significantly lower than that in O group((54.35± 3.66)s,(46.31±4.81)s,(36.81±3.86)s).Mice in HPC+O spent longer time in the target quadrant than that in O group((36.44±5.33)%and(24.5±2.59)%,respectively, P<0.05).Immunofluorescence showed that the apoptotic ration of nerve cells in hippocampal CA 1 was significantly lower than that in O group ( 11.7 ± 0.14 and 1.35±0.14, P<0.05).Conclusion Hypoxic preconditioning can increase hippocampal CA1 neurons hypoxia tolerance of ischemia reperfusion injury in mice,and reduce the incidence of neural cell apoptosis.
