Ultrasound-mediated microbubbles destruction enhances the transplantation of endothelial progenitor cells for improvement of chronic allograft vasculopathy in rats
10.3760/cma.j.issn.1004-4477.2016.01.017
- VernacularTitle:超声介导微泡在内皮祖细胞移植修复大鼠慢性移植物血管病中的应用
- Author:
Ji MA
;
Suya MA
;
Xianxian YUAN
;
Ye FANG
;
Feng CHEN
- Publication Type:Journal Article
- Keywords:
Sonication;
Microbubbles;
Endothelial progenitor cells;
Chronic allograft vasculopathy
- From:
Chinese Journal of Ultrasonography
2016;(1):75-80
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of using ultrasound‐mediated destruction of microbubbles ( US+ MB) to enhance the transplantation of endothelial progenitor cells ( EPCs) to confer chronic allograft vasculopathy (CAV) .Methods Bone marrow derived mononuclear cells were isolated and induced in vitro . The abdominal aorta transplantation was performed . Four groups were divided:control group without treatment (group A) ,injection with saline (group B) ,injection with EPCs (group C) ,group D ( US+MB+EPCs) was injected with EPCs and US was applied to MB prior to the infusion . All rats were killed during 8 weeks after transplantation to enable histological examination;SDF‐1α expression was detected by immunohistochemistry ,the expression of SDF‐1αand TNF‐αin the grafted aortas were detected with RT‐PCR . Results When 8 weeks after EPCs transplantation ,there was a significant improvement in aortic intima of Group D compared with Group B and C ,respectively ( P <0 .05) . In addition ,treatment of Group D significantly increased the expression of SDF‐1αand reduced the expression of TNF‐αin the grafted aortas . Conclusions US‐mediated MB destruction prior to EPCs transplantation into the grafted aortas can improves the effectiveness of endothelial repair and delay the progress of CAV .
