Effects of New Tangshenkang on α-SMA and E-cadherin of Human Renal Tubular Epithelial Cell HK-2 in High Concentrations of Glucose
10.3969/j.issn.1005-5304.2016.03.015
- VernacularTitle:新加糖肾康对高糖环境下人肾小管上皮细胞α-平滑肌肌动蛋白和 E-钙黏蛋白的影响
- Author:
Miaorui ZHU
;
Zhuo QUAN
;
Lixia YANG
;
Tao CHENG
;
Dinghua ZHANG
;
Hanyuan GAO
;
Wen SUN
- Publication Type:Journal Article
- Keywords:
new Tangshenkang;
high glucose;
human renal tubular epithelial cell HK-2;
epithelial-myofibroblast transdifferentiation;
cell proliferation
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(3):54-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe he effects of new Tangshenkang on α-SMA and E-cadherin of human renal tubular epithelial cell HK-2 in high concentrations of glucose; To explore the mechanism of new Tangshenkang on the prevention and treatment of diabetic renal fibrosis. Methods The HK-2 cells were cultured and divided into control group, high glucose group, animal serum control group, new Tangshenkang low-, medium-, and high-dosage group. After medicine intervention, cell proliferation was tested by MTT assay, and contents of α-SMA and E-cadherin were observed by ELISA assay. Results Compared with control group, α-SMA of HK-2 cultured with high glucose was much notable, but the content of E-cadherin significantly decreased, with statistical significance (P<0.05). The content of α-SMA of HK-2 cultured with new Tangshenkang decreased, and the content of E-cadherin increased; cell proliferation was markedly inhibited in culture medium supernatant of HK-2 cells cultured with high glucose plus new Tangshenkang compared with only high glucose, with statistical significance. Conclusion New Tangshenkang can inhibit cell proliferation and epithelial-myofibroblast transdifferentiation of HK-2 cell induced by high glucose, and prevent the development of diabetic renal fibrosis to a certain extent.
