Determination of Mahuannin A in Ephedrae Radix et Rhizoma by HPLC-ELSD
- VernacularTitle:HPLC-ELSD法测定麻黄根药材中麻黄宁A的含量
- Author:
Dongyan CHENG
;
Lishu WANG
;
Jun GAO
;
Donghong CHENG
;
Chaonan WANG
- Publication Type:Journal Article
- Keywords:
Ephedrae radix etrhizaoma;
Mahuannin A;
HPLC-ELSD
- From:
China Pharmacist
2016;19(2):360-361
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC-ELSD method for the determination of mahuannin A in ephedrae radix et rhizoma. Methods:The content of mahuannin A was determined by an HPLC-ELSD method on an Alltima TM C18 column (250 mm × 4. 6 mm, 5 μm). The mobile phase was acetonitrile-water (28∶ 72) with a flow rate of 0. 7 ml·min-1, and the column temperature was 30℃. The temperature of drift tube heater was 105℃ and the flow rate of carrier gas was 2. 8 L·min-1 . Results:The linear range of mahua-nnin A was 42. 56-383. 04 μg·ml-1(r=0. 999 8). The average recovery and RSD was 99. 9% and 1. 96%(n=6), respectively. Conclusion:The method is simple and the result is accurate. It can be used for the quality control of ephedrae radix et rhizaoma.
