Comparison of Two Methods for the Determination of Ciprofloxacin in Compound Cod-liver Oil Emulsion
- VernacularTitle:两种不同方法测定复方鱼肝油乳剂中环丙沙星含量的比较
- Author:
Xiang LI
;
Bei HU
;
Ming YAN
;
Hongda MA
- Publication Type:Journal Article
- Keywords:
Ciprofloxacin;
HPLC;
UV spectrophotometry
- From:
China Pharmacist
2016;19(2):362-364
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare two methods for the determination of compound cod-liver oil emulsion. Methods:The content of ciprofloxacin in compound cod-liver oil emulsion was determined by HPLC and UV, respectively. The determination by HPLC was performed on a Thermo C18 column (150 mm ×4.6 mm, 5 μm). The mobile phase was 0.025 mol·L-1phosphate-acetonitrile (87∶13)andpHwasadjustedto3.0±0.1withtriethylamine. Thedetectionwavelengthwas277nmandtheflowratewas1.5ml·min-1. The detection wavelength of UV was 277 nm. Results:The average recovery of HPLC and UV was 100. 44% and 100. 84% with RSD of 1. 01% and 1. 09% (n=9), respectively. The detection results of the two methods were compared by paired sample t-test, and no statistically significant difference was found (P>0. 05). Conclusion:The two methods are specific and accurate, and can be used for the determination of ciprofloxacin hydrochloride in compound cod-liver oil emulsion.
