NF-κB regulates apoptosis of human alveolar epithelial cells induced by MRP8/14
10.3969/j.issn.1000-4718.2016.04.020
- VernacularTitle:NF-κB介导M RP8/14诱导的人肺泡上皮A549细胞凋亡
- Author:
Lin HUANG
;
Chen YANG
;
Jie LIANG
;
Haihua LUO
;
Yong JIANG
;
Aihua LIU
- Publication Type:Journal Article
- Keywords:
Acute lung injury;
Apoptosis;
Myeloid-related protein 8/14;
Alveolar type Ⅱ epithelial cells;
Nuclerar factor-κB
- From:
Chinese Journal of Pathophysiology
2016;32(4):701-706
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of myeloid-related protein 8/14 ( MRP8/14 ) on the survival and apoptosis of human alveolar epithelial cell line A549, and to explore the role of nuclear factor-κB (NF-κB) in this process. METHODS:A549 cells were treated with different doses of MRP8/14 or stimulated with MRP8/14 for different time.The effect of MRP8/14 on the viability of A549 cells was determined by CCK-8 assay.The apoptotic rates were tested by flow cytometry.The nuclear translocation of NF-κB p65 was detected by Western blot and indirect immunofluorescence.Be-sides, the phosphorylation level of NF-κB p65 was determined by Western blot.NF-κB-specific inhibitor Bay 11-7082 was used to further analyze the role of NF-κB in the apoptosis induced by MRP8/14.RESULTS:The viability of the A549 cells was affected by MRP8/14 in a dose-and time-dependent manner.Flow cytometric analysis showed that the apoptotic rates were increased in the cells treated with MRP8/14 (P<0.01).In A549 cells administered with MRP8/14, NF-κB p65 was significantly phosphorylated and translocated into the nuclei, suggesting the activation of NF-κB signaling pathway. However, NF-κB-specific inhibitor Bay 11-7082 significantly attenuated the cell apoptosis induced by MRP8/14 ( P <0.01).CONCLUSION:NF-κB plays an important role in regulating the apoptosis of human alveolar epithelial cells in-duced by MRP8/14.
