Influence of stem-like cells on EMT occurrence in mice with triple-negative breast can-cer and on their biological behavior
10.3969/j.issn.1000-8179.2016.08.003
- VernacularTitle:小鼠三阴性乳腺癌干样细胞对EMT发生及其生物学行为的影响
- Author:
Jiameng LIU
;
Baocun SUN
;
Huizhi SUN
;
Danfang ZHANG
;
Xian LIN
;
Yanlei LI
;
Qiang GU
;
Xueyi DONG
;
Fang LIU
- Publication Type:Journal Article
- Keywords:
triple-negative breast cancer;
epithelial-mesenchymal transition;
cancer stem-like cells;
aldehyde dehydrogenase 1;
CD133;
flow cytometry
- From:
Chinese Journal of Clinical Oncology
2016;43(8):324-328
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To discuss the influence of ALDH1+and CD133+phenotypic breast cancer stem-like cells in TA2 triple negative breast cancer on promoting epithelial-mesenchymal transition (EMT) occurrence in TA2 mice with triple-negative breast cancer and on their biological behavior. Methods:Flow cytometry was performed to analyze the markers ALDH1 and CD133 in TA2 mice triple nega-tive breast cancer and breast cancer stem-like cells with ALDH1+, ALDH1?, CD133+, and CD133?phenotypes, which were sorted out. Then, the TA2 mice were inoculated with sorted tumor cells according to cell type. The mice were divided into ALDH1+, ALDH1?, CD133+, and CD133-groups. The tumor-growing conditions were observed. A tumor tissue was sliced for the immunohistochemical testing of ALDH1?, CD133?, and EMT-related Twist1, E-cadherin, and VE-cadherin proteins. The expression difference of breast cancer stem cell surface markers ALDH1 and CD133 in triple-negative breast cancer and EMT-related proteins Twist1, E-cadherin, and VE-cad-herin was analyzed. Results:The expression rates of breast cancer stem cell markers ALDH1 and CD133 in TA2 mice triple negative breast cancer were 31.2%and 6.5%, respectively. The tumor growth ability of TA2 mice from ALDH1+group was obviously stronger than that from ALDH1?group. The CD133+group was evidently stronger than CD133?group. The immunohistochemical results showed that ALDH1, Twist1, and VE-cadherin expression levels in the ALDH1+group were evidently higher than that in the ALDH1?group (all P<0.05). E-cadherin expression decreased (P<0.05). CD133?, Twist1, and VE-cadherin expression levels in CD133+group were higher than that in CD133?group (all P<0.05). Conclusion:In TA2 mice triple negative breast cancer, ALDH1+and CD133+phenotypic breast cancer stem-like cells may influence the expression of EMT-related proteins, and promote the formation of triple-negative breast cancer.
