Simultaneous Determination of Four Kinds of Component Contents in Litong Pill by Double-wavelength HPLC
10.3870/j.issn.1004-0781.2016.05.021
- VernacularTitle:双波长高效液相色谱梯度洗脱法同时测定黎峒丸中4种成分含量
- Author:
Shenyan KONG
;
Xian LI
- Publication Type:Journal Article
- Keywords:
Litong pill;
Neogambogic acid;
Gambogic acid;
( +)-Catechin;
L-epicatechin;
Cutti CamBogia;
Catechu;
Determination
- From:
Herald of Medicine
2016;35(5):514-517
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a double-wavelength HPLC method for the simultaneous determination of neogambogic acid,gambogic acid,( +)-catechin and L-epicatechin in Litong pill. Methods The quantitative analysis was carried out on C18 column(250 mm×4.6 mm,5 μm).A linear elution of methanol-acetonitrile(4: 1)and 0.2% phosphoric acid solution was adopted at the flow rate of 1.1 mL·min-1 ,with the detection wavelength set at 360 nm for neogambogic acid and gambogic acid,and at 280 nm for(+)-catechin and L-epicatechin. Results The linear ranges of neogambogic acid,gambogic acid,(+)-catechin and L-epicatechin were 5.85-117.00 μg·mL-1(r= 0.999 5),8.95-179.00 μg·mL-1(r= 0.999 8),6.90-138.00 μg·mL-1(r= 0.999 7),and 5.30-106.00 μg·mL-1(r = 0.999 9),respectively.The average recoveries were 97.82%(RSD= 1.21%),98.72%(RSD = 1.30%),96.96%(RSD = 0.84%)and 99.26%( RSD = 1.46%)for neogambogic acid, gambogic acid,(+)-catechin and L-epicatechin,respectively. Conclusion The method is simple,accurate,reproducible and may be used for the simultaneous determination of neogambogic acid,gambogic acid,(+)-catechin and L-epicatechin in Litong pill.
