Periodontal regeneration on surface of tooth root using BMSCs sheet combined with a sandwich structure:a canine model in situ
10.7644/j.issn.1674-9960.2016.04.007
- VernacularTitle:利用骨髓间充质干细胞膜片原位构建“三明治”样组织工程牙周膜的实验研究
- Author:
Cui LIU
;
Biao ZHU
;
Xinran ZHANG
;
Donghua LIU
;
Gang ZHAO
;
Ximin GUO
;
Hongyan GUO
- Publication Type:Journal Article
- Keywords:
bone marrow mesenchymal stem cell;
cell sheet;
periodontal ligament regeneration;
extracellular matrix;
tissue engineering
- From:
Military Medical Sciences
2016;40(4):294-298
- CountryChina
- Language:Chinese
-
Abstract:
Objective To probe the periodontal ligament regeneration following the implantation of bone marrow mesenchymal cells ( BMSCs ) sheet-collagen membrane-BMSCs sheet sandwich complex.Methods BMSCs cell sheet-collagen membrane-BMSCs sheet complexes were compounded on the root surface of teeth of Beagle dogs.All the dogs were killed on 4 and 12 weeks after implantation.Periodontal ligament regeneration was observed by radiological means, HE staining and Sirus-red staining.Results Compared with collagen membrane group and blank control group, there was a clearly periodontal ligament like tissue and Sharpey′s like fibres formation in test group only.Conclustion Cell sheet-collagen membrane-cell sheet sandwich complex can effectively improve the periodontal ligament regeneration.
