Regulation of AT1-calcineurin Signaling Pathway on Transient Outward Potassium Ion Channel Remolding in Hypertrophic Atrial Myocytes of Neonatal Rats
10.3969/j.issn.1000-3614.2016.04.020
- VernacularTitle:血管紧张素受体1型-钙调神经磷酸酶信号通路对肥大乳鼠心房肌细胞瞬时外向钾电流离子通道重构的调控作用
- Author:
Guiling XIA
;
Yanan XU
;
Long YANG
;
Jun LI
;
Jionghong HE
;
Na DENG
;
Longhai TIAN
;
Yin TIAN
- Publication Type:Journal Article
- Keywords:
Receptors,angiotensin type I;
Ion channels;
Signaling transduction;
remodeling;
Calcineurin
- From:
Chinese Circulation Journal
2016;31(4):398-402
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the role of angiotensin receptor type I (AT1)-calcineurin (CaN) signaling pathway in transient outward potassium ion channel (Ito) remolding in hypertrophic atrial myocytes of neonatal rats. Methods: 1 day old neonatal rats’ atrial myocytes were isolated and the cells were divided into 4 groups:①Control group, normal cells were cultured for 24 h,②Stretching group, the cells were cultured for 24 h with mechanical stretching to induce hypertrophy,③Telmisartan group, the cells were treated by telmisartan at 1 μmol/L for 1 h, then cultured for 24 h and ④Cyclosporin-A (CsA) group, the cells were treated by CsA at 0.25 μg/ml for 1 h, then cultured for 24 h. The ratios of protein/DNA in myocytes were compared between Control group and Stretching group, cell hypertrophy was deifned by mRNA expression of atrial natriuretic peptide (ANP). Ito changes were detected by whole-cell patch clamping technique, proteins expressions of Kv4.3 and CaN A subunit were examined by Western blot analysis. Results: Compared with Control group, Stretching group showed obviously decreased Ito density and Kv4.3 protein expression, while increased CaN A protein expression; Compared with Stretching group, the above effects were reduced in Telmisartan group and CsA group. Conclusion: AT1-CaN signaling pathway was involved in the regulation of Ito channel remodeling in hypertrophic atrial myocytes of neonatal rats.
