Experimental study of WT1 specific CD8+T cells in the treatment of breast cancer
10.11958/20150353
- VernacularTitle:WT1特异性CD8+T细胞治疗乳腺癌的实验研究
- Author:
Xinchao WANG
;
Suhong HAO
;
Yingtang GAO
;
Lijun QIU
;
Shuang ZHAO
;
Lu HAN
- Publication Type:Journal Article
- Keywords:
breast neoplasms;
immunotherapy,adoptive;
CD8-positive T-lymphocytes;
Wilms’tumor gene 1;
cell im-munotherapy
- From:
Tianjin Medical Journal
2016;44(4):397-400
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of Wilms’tumor gene 1 (WT1)-specific CD8+T cells from periph?eral blood for the treatment of breast cancer by detecting the killing activity of WT1 specific CD8+T cells on breast cancer cells. Methods Flow cytometry was used to detect WT1-specific CD8+T cells in the peripheral blood of 20 samples from HLA-A2 seropositive healthy donors, which were isolated by WT1/MHC streptamer magnetic beads and cultured. The func?tion of WT1-specific CD8+ T cells were analysis by cytotoxicity assay. Results Twelve of 20 healthy donors had naive WT1-specific CD8+T-cell frequencies of>0.5%, and 4 of 20 even>1.0%of all CD8+T cells. After positive selection by magnetic cell separation, a purity of up to 80%can be achieved. WT1 specific CD8+T cells can specifically kill breast can?cer cell line with WT1 polypeptide. Conclusion WT1 specific CD8+T cells can be detected in peripheral blood of healthy volunteers. WT1 specific CD8+T cells have killing effect on breast cancer cells, suggesting the feasibility of adoptive immu?notherapy for breast cancer.
