Research of in vitro Anti-tumor Effect of Hydroxychloroquine
- VernacularTitle:羟基氯喹体外抗肿瘤活性研究
- Author:
Tao YANG
;
Guoming LI
;
Linlu XUE
;
Bo HUANG
;
Qi WANG
;
Changsheng DENG
;
Qin XU
;
Jianping SONG
- Publication Type:Journal Article
- Keywords:
Hydroxychloroquine;
Anti-tumor activity;
In vitro
- From:
China Pharmacist
2016;19(3):417-419
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate and evaluate the anti-tumor activity of hydroxychloroquine ( HCQ) in vitro. Methods:CCK-8 assay was applied to detect the inhibitory effect of HCQ at different concentrations(4. 78, 9. 55, 19. 10, 38. 20,76. 40 μg?ml-1 ) on A549 cells, HepG2 cells, HT-29 cells, K562 cells, Hela cells and B16 cells. Absorbance was detected by a microplate reader, and then the inhibitory rate of the tumor cells and the IC50 was calculated. Results:Compared with those of the negative control group, the inhibitory rates of HCQ at different concentrations against the six tumor cells were all increased significantly in a dose-dependent manner (P<0. 01). The inhibitory rates of the tumor cells were all above 60% when the concentration reached 38. 20μg?ml-1. The IC50 was 26. 70(A549), 27. 47(HepG2), 5. 72(HT-29), 14. 03(K562), 20. 21(Hela) and 13. 62(B16) μg?ml-1, respectively. Conclusion:HCQ shows anti-tumor activity on several cancer cells in vitro. There may be a good application prospect for HCQ in the treatment of colorectal cancer, liver cancer, cervical cancer, melanoma and leukemia.
