Plasma Proteomic Analysis of Early Diabetic Nephropathy Patients with Kidney Yang Deficiency
10.13359/j.cnki.gzxbtcm.2014.06.008
- VernacularTitle:早期糖尿病肾病肾阳虚证的血浆蛋白质组学分析
- Author:
Hongmei LU
;
Shuifu TANG
;
Kunhua HU
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathy;
Kidney yang deficiency;
Proteomics;
Two-dimensional fluorescence difference gel electrophoresis
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2014;(6):890-894
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the sensitive plasma molecular markers in diabetic nephropathy ( DN) patients with kidney yang deficiency. Methods Two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) was used to analyze the plasma which was sampled from 4 early DN patients with kidney yang deficiency and 4 healthy adult volunteers. Proteins that showed differential expression with an over 1.5-fold change were analyzed by MALDI-TOF/TOF mass spectrometry. Results Two-dimensional electrophoresis (2-DE) maps of plasma proteins in DN patients with kidney yang deficiency and healthy adults were established successfully. We validated 9 differentially expressed proteins, including complements C3 and C4, apolipoprotein E and ubiquitination factor. Conclusion Proteomic analysis by 2-D DIGE can objectively reveal the differences of plasma protein expression in DN patents with kidney yang deficiency and healthy adult volunteers. The obtained 9 proteins have potential to provide plasma molecular markers for the early clinical diagnosis and for the research of traditional Chinese medical patterns of DN.
