Preparation of polyclonal antibody for minoxidil, development of an ELISA method for detection of minoxidil
10.3969/j.issn.1000-484X.2015.12.018
- VernacularTitle:米诺地尔多克隆抗体的制备及其酶联免疫检测方法的建立
- Author:
Biao WEI
;
Guirong ZHANG
;
Danshen ZHANG
;
Lingyun NIE
;
Yanping TANG
- Publication Type:Journal Article
- Keywords:
Minoxidil;
Artificial antigen;
Polyclonal antibody;
ELISA
- From:
Chinese Journal of Immunology
2015;31(12):1668-1673
- CountryChina
- Language:Chinese
-
Abstract:
Objective:On the basis of synthetic antigens on minoxidil ,we prepared its polyclonal antibody and established a ELISA method to detect minoxidil .Methods:We synthesized minoxidil artificial antigen with glutaraldehyde and identified by UV scan -ning.We had established a detection of minoxidil competition ELISA .Results: The UV scan showed that the minoxidil successfully coupled to a carrier protein.The conjugate of minoxidil-BSA was used to immunize BALB/c mice.And the produced antiserum showed high titer of 1:12 800 in the indirect ELISA.Its ELISA curve equation:y=-0.082 3 x+0.938 ( R2 =0.9811 ) , minoxidil mass concentration and the absorbance in 0.001-10 μg/ml showed a good linearity .Conclusion: This method has been successfully preparing minoxidil artificial antigen and polyclonal antibodies and established ELISA detection method for minoxidil ,which provides a basis for the practical application of minoxidil immunoassay method .
