IL-4 induced proliferation in prostate cancer PC3 cells via regulating LDH-A expression

VernacularTitle:白细胞介素－4调控糖代谢相关基因LDH-A诱导前列腺癌细胞PC3的增殖
Author: Zhiduan CAI ; Guo CHEN ; Xin FU ; Jianheng YE ; Bowei LI ; Minyao JIANG ; Weide ZHONG
Publication Type:Journal Article
Keywords: IL-4; LDH-A; prostate cancer cells; proliferation
From: The Journal of Practical Medicine 2016;32(4):516-519
CountryChina
Language:Chinese
Abstract: Objective To study the effects of IL-4 in prostate cancer cells glycometabolism and proliferation. Methods We used IL-4 to treat PC3 cells, then tested the changes of LDH-A expression by RT-qPCR, Western Blot, CCK-8 and lactate production assay. Results Our data showed that IL-4 induced LDH-A up-expression in PC3 cells at mRNA and protein levels. Also, IL-4 promoted the proliferation activation and increased lactate production in PC3 cells. Conclusion IL-4 can strengthen the proliferation activation in PC3 cells by up-regulating LDH-A expression.