Vasodilatory effect of Ferulic acid on in-vitro rat coronary artery
10.3969/j.issn.1001-1978.2016.04.022
- VernacularTitle:阿魏酸对离体大鼠冠状动脉的舒张作用及机制探讨
- Author:
Longmei FANG
;
Xiaomin HOU
;
Rong YANG
;
Fangwen FAN
;
Zefang HE
;
Meng SHI
;
Mingsheng ZHANG
- Publication Type:Journal Article
- Keywords:
FA;
in vitro coronary artery;
vasodilator effect;
mechanism;
vascular ring;
K+ channel;
Ca2+channel
- From:
Chinese Pharmacological Bulletin
2016;32(4):554-558
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the vasodilatory effect of Ferulic acid on in vitro rat coronary artery and its possible mechanism. Methods By using the mi-crovessel tension recorder system, the vasodilatory effect of FA on resting and contractin-vitro rat coronary artery was determined;the influence of endothelial in-tegrity to FA-induced vasorelaxation was observed; the relationship of FA on [ Ca2+] ex-influx-induced and [ Ca2+] in-efflux-induced contractions was discussed;the mechanism of vasodilatory effect of FA was ex-plored by applying the inhibitors of KCa(TEA),KATP channel ( Gli ) , KIR channel ( BaCl2 ) , KV ( 4-AP ) , NOS( L-NAME) and COX( Indo) . Results FA had no effect on the resting tension of in vitro rat coronary artery. FA dilated the in-vitro rat coronary artery pre-treated with KCl ( 60 mmol · L-1 ) , U46619 ( 1 μmol · L-1 ) and PE ( 10μmol · L-1 ) in a concentration-dependent fashion ( P < 0. 05 ) . FA inhibited the [ Ca2+] ex-influx-induced and [ Ca2+] in-efflux-in-duced contractions significantly ( P <0. 05 ) . 4-AP ( 1 mmol· L-1 ) restrained the diastolic function of FA, while TEA, Gli, BaCl2、L-NAME, Indo had no obvious effect ( P >0. 05 ) . Conclusion The diastolic func-tion could be related to the activation of KV channel on vascular smooth muscle cells, the free Ca2+ from Sar-coplasmic reticulum cells and blockade extracellular Calcium channel do not depend on KCa, KATP, KIR channel, nor the endothelial function.
