Interleukin-15 enhanced the cytotoxicity of Vδ2 γδ T cells against K562 cells
10.3760/cma.j.issn.0254-5101.2015.11.004
- VernacularTitle:白细胞介素15促进 Vδ2γδT 细胞杀伤人白血病 K562细胞
- Author:
Wenwei YIN
;
Qiongfang ZHANG
;
Jianying SHAO
;
Shiwen TONG
- Publication Type:Journal Article
- Keywords:
IL-15;
Vδ2 T cell;
IFN-γ;
K562 cell
- From:
Chinese Journal of Microbiology and Immunology
2015;(11):812-815
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of interleukin -15 ( IL-15 ) on the cytotoxicity of Vδ2 γδ(Vδ2) T cells against K562 cells.Methods PBMCs were separated and cultured with zoledronate and interleukin-2 ( IL-2) to induce the proliferation of Vδ2 T cells.The obtained Vδ2 T cells were in vitro stimulated with IL-15.Flow cytometry analysis was performed to evaluate the changes of phenotypes of Vδ2 T cells and their cytotoxicity against K562 cells.Results Zoledronate effectively induced the massive prolifer -ation of V2δT cells.The Vδ2 T cells were highly activated and the expression of CD 107a and IFN-γby Vδ2 T cells were upregulated upon IL-15 stimulation.The cytotoxicity of Vδ2 T cells against K562 cells was greatly enhanced by the treatment with IL-15.Conclusion IL-15 could activate the Vδ2 T cells and en-hance their cytotoxicity against K562 cells.
